Role of ProEx C immunocytochemistry in cervical high-grade squamous intraepithelial lesions detection

Vol. 62 No. 4, 2021


Carmen Ungureanu, Demetra Gabriela Socolov, Gabriela Anton, Eugenia Morosan, Laura Mihaela Trandafir, Ludmila Lozneanu, Mioara Florentina Trandafirescu, Elena Cojocaru

Background: Screening programs using Papanicolaou smear along with human papillomavirus (HPV) testing led to a significantly decrease of cervical cancer rates. Nevertheless, both assessments have limited specificity for revealing cervical high-grade lesions. The main problem is how to identify the real precursor of cervical squamous cell carcinomas (SCC), namely high-grade squamous intraepithelial lesions (HSIL). Aim: The aim of our study was to conclude if ProEx C might be used as a marker for high-grade cervical intraepithelial neoplasia (CIN). Materials and Methods: In this study, we detected the immunochemical expression of anti-ProEx C antibody in liquid-based cytology (LBC) samples. We analyzed a total number of 125 cervical cytology specimens. Results: In 48% of all cases, ProEx C was found to be positive. The percentage increased from 0% in negative for intraepithelial lesion or malignancy (NILM) cases to 100% in SCC cases. Conclusions: ProEx C may be utilized to improve the accuracy of cytological diagnosis on cervical smears, according to the findings of this study. This marker is also useful in detecting unrevealed high-grade lesions on atypical squamous cells of undetermined significance (ASC-US), low-grade squamous intraepithelial lesions (LSIL) smears, being very useful in establishing the conduct of these cases.

Corresponding author: Ludmila Lozneanu, Lecturer, MD, PhD; e-mail:; Mioara Florentina Trandafirescu, Lecturer, MD, PhD; e-mail:

DOI: 10.47162/RJME.62.4.15 Download PDF
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