Assessment of hormonal receptor immunoexpression and Her2 status in invasive breast cancer after conservative and radical surgery

Vol. 59 No. 3, 2018


Tiberiu Stefanita Tenea-Cojan, Adrian Macovei, Ion Paun, Andrei Ionut Costin, Claudia-Valentina Georgescu, Corneliu Cristian Georgescu, Ionela Mihaela Vladu, Cristina Gabriela Ene, Lucretiu Radu

This study was conducted on 300 patients with infiltrative breast carcinoma, of which 112 cases underwent radical mastectomy, and 188 were conservatively operated. Forty-four patients experienced local relapses during the clinical follow-up. The immunohistochemical (IHC) study used the Labeled Streptavidin Biotin (LSAB)/Horseradish peroxidase (HRP) method, which evaluated the estrogen receptor (ER) and progesterone receptor (PR), as well as human epidermal growth factor receptor 2 (Her2/neu) expressions in tumor cells. After IHC analysis, all cases were classified into one of the following four immunophenotypes: ER+/PR+ classical phenotype, ER-/PR-, ER+/PR- and ER-/PR+ phenotypes. Correlating hormonal phenotypes with Her2 status, we found that Her2 is significantly associated more frequently with the ER+/PR- phenotype, while the absence of Her2 was associated more frequently with the ER+/PR+ phenotype but with no significant differences. Local recurrences were significantly more common in patients with absent ERs than those who had these receptors present in the primary tumor. Similarly, mammary tumors with absent PRs recurred significantly more frequently than those with PRs. The positivity of Her2 is significantly associated with the absence of PRs in both type of surgery (conservative or radical). Local recurrences are significantly correlated with both negative receptors phenotype, regardless of the type of surgery. IHC markers, represented by hormone receptors for estrogen and progesterone, and Her2 oncoprotein, can be useful for identifying patients who are at increased risk of local recurrences after conservative or radical surgery for breast cancer.

Corresponding author: Andrei Ionut Costin, MD, PhD Student; e-mail:; Adrian Macovei, Teaching Assistant, MD, PhD; e-mail:

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