Placenta changes in pregnancy with gestational diabetes

Vol. 57 No. 2 Suppl., 2016
This supplement was not sponsored by Outside Organizations.

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Antoine Edu, Cristina Teodorescu, Carmen Gabriela Dobjanschi, Zita Zsuzsana Socol, Valeriu Teodorescu, Alexandru Matei, Dinu Florin Albu, Gabriela Radulian

Placental damage may be responsible for the fetal complications in pregnancies complicated by diabetes. We have analyzed the prevalence of gestational diabetes (GD) in a population of 109 pregnant women, the risk factors and the placental changes associated with gestational diabetes. Tests carried out were oral glucose tolerance test at 24-28 weeks of gestation, using the IADPSG (International Association of Diabetes and Pregnancy Study Groups) criteria for gestational diabetes, glycated hemoglobin, fasting insulin, total cholesterol, high density lipoprotein (HDL)-cholesterol, low density lipoprotein (LDL)-cholesterol, triglycerides, two-dimensional (2D) ultrasound and, also, there were analyzed macro and microscopic placental fragments from pregnant women with/without GD. It has been recorded the weight of placenta at birth and there were analyzed the possible pathological changes. The prevalence of GD was 11.9%. We have applied the direct logistic regression to determine the impact of some factors over the probability of association with gestational diabetes. The most powerful predictor was the placental maturity grade, the patients with decreased maturity grade having chances 52.6 times higher than those with an increased placental maturity grade to associate gestational diabetes. Sizes of placentas in patients with gestational diabetes mellitus were significantly increased than in patients without this diagnosis (p=0.012) from week 24-28. Pathological changes were discovered in six of the 13 placentas of women with gestational diabetes mellitus, independent of the level of glycated hemoglobin (p=0.72). The level of hyperglycemia is only partially associated with the presence of placental changes, which may be caused by other maternal factors.

Corresponding author: Carmen Gabriela Dobjanschi, MD, PhD; e-mail: dgcarmen2004@yahoo.com

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ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Georgiana Gabriela Petrica-Matei, Florin Iordache, Razvan Hainarosie, Marinela Bostan

Although it is very difficult to establish a correlation between the behavior of tumor cells in vitro and in vivo tumors, in this study we tried to analyze some functions of the tumor cells isolated from head and neck tumor fragments, comparatively with the tumor cell cultures, hoping that the results could help the clinician to choose optimal treatment for head and neck cancer patients. The aim of the present study was to evaluate the expression of p53 protein, the apoptosis process and the cell cycle parameters in the cell cultures of head and neck carcinoma obtained from fragments of tumor excised from patients. Using ELISA assay and flow cytometry methods we analyzed the p53 protein expression, distribution of the cell cycle phases and the level of apoptosis in the tumor cells which were immediately isolated from the tumor fragments, as well as in the ones that were subsequently passed in culture several times. Our results showed that p53 protein expression was weak in four (33.3%) patients, moderate in three (25%) patients and intense in five (41.6%) patients. Our data showed that both tumor cells isolated from the tumor fragments (passage 0), as well as cell cultures at passage 5 had the percentage of S-phase of the cellular cycle higher than 20%, suggesting that tumors have high proliferative activity. The tumor cells cultures have a small percentage of apoptosis that may reflect a biological aggressive tumor character. In conclusion, these data may improve the diagnostic protocols and help to an efficient monitoring of therapy.

Corresponding author: Marinela Bostan, Research Scientist III, PhD; e-mail: marinela.bostan@yahoo.com

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