An application of microscopic investigation on extracted premolar with dyschromia, related to the components of endodontic sealer

Vol. 57 No. 2 Suppl., 2016
This supplement was not sponsored by Outside Organizations.

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Ioana Suciu, Ecaterina Ionescu, Costin Varlan, Oana Elena Amza, Sinziana Adina Scarlatescu, Ileana Suciu, Mihai Ciocirdel, Bogdan Alexandru Dimitriu

The aim of this study was an assessment of the overall optical characteristics changes in volume of the dentin, after the diffusion of the chemical components included in the sealer throughout the endodontic space, in order to demonstrate the discoloration effect of the endodontic sealer on tooth crown of a recently extracted maxillary premolar. Thin sections were studied using the polarized transmitted light microscopy and under a stereomicroscope, operating in transmitted light and reflected light. There were performed serial images of magnitude 40x. Each image was covered by a grid aimed for microscopic sections volumetric measurements. Therefore, all the serial images were merged and resulted a global image of the entire section surface. Thus, we have analyzed a total of three sections of the same tooth. Based on these sets of images, there were made estimations of the areas affected by colorations, determinations that can be extrapolated to total tooth areas, in terms of volumetric color change of dentin. The proportion of low coloristic infiltrated dentin was very close to the deeply impregnated dentin and the overall impregnated dentin covered half of the total dentin analyzed area.

Corresponding author: Ioana Suciu, Associate Professor, DMD, PhD; e-mail: joa_suciu@yahoo.com

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ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Oana Claudia Deleanu, Ana-Maria Zaharie, Aneta Serbescu, Floarea Mimi Nitu, Florin Dumitru Mihaltan, Oana-Cristina Arghir

The features of bronchoalveolar lavage fluid (BALF) components and the severity of pulmonary alveolar proteinosis (PAP) were analyzed in the first Romanian cohort of patients admitted to Marius Nasta Institute of Pneumophtisiology, Bucharest, Romania. A six-year follow-up study based on bronchoalveolar lavage (BAL) data was performed between January 2007 to December 2012. Study cohort consisted in 20 inpatients diagnosed with PAP, based on BALF cytological findings and/or on histopathological findings. Demographic, medical history, tobacco use, clinical and radiological features, disease progression with or without whole lung lavage (WLL) therapy were collected. Disease severity was evaluated by pulmonary function testing including spirometry, blood gas analysis, plethysmography, and diffusing capacity of the lung for carbon monoxide (DLCO), also known as transfer factor (TLCO). The cellular profile of all BALF specimens was analyzed. Statistical analysis made by SPSS version 17.0 included Student s t-test, chi-square test and ANOVA. Mean age of the subjects was 43+/-16.59 years, with male predominance (n=12; 60%). Diagnosis of PAP was facilitated by fiberbronchoscopy (FBS) with BAL in 90% of cases. Cytological findings of BALF revealed lower macrophages (57.26+/-18.19%), with a preponderance of neutrophils (17.75+/-19.44%) and lymphocytes (21.8+/-16.12%). Lower oxygen partial pressure was identified in elders, comparing to younger patients (p=0.038). Patients treated by WLL had a lower total lung capacity (TLC) and DLCO versus those who did not required WLL (p=0.009, respectively p=0.056). The severity of pulmonary abnormalities provided WLL indication was not influenced by BALF cellularity.

Corresponding author: Ana-Maria Zaharie, MD, PhD Student; e-mail: ana_neb@yahoo.com

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