The histopathological study of radicular dentinal changes in patients with chronic marginal periodontopathies

Vol. 56 No. 2 Suppl., 2015
This supplement was not sponsored by Outside Organizations.


Melania Olimpia Cojocaru, Elvira Florina Patroi, Dorina Corlan Puscu, Mirela Lucia Opri, Monica Mihaela Craitoiu, Laurentiu Mogoanta, Evantia Coles, Vasile Nicolae, Mariana Sabau

Periodontal disease is one of the most frequent conditions in individuals, having major health and social implications. Progressing as a chronic inflammation at the level of tooth support tissues, untreated chronic periodontitis may lead to a premature loss of the dental organ. Even though the main lesions caused by the periodontal disease affect the alveolo-dental ligaments, we tried to highlight the presence of certain lesions of radicular dentine in patients with chronic marginal periodontopathy. In our study, in the patients with chronic marginal periodontopathy, at periodontium level, there was highlighted the presence of a chronic inflammatory process with numerous lymphocytes and macrophages, microhemorrhages, and also areas with tissular necrosis. At radicular dentine level, in the cases of old, untreated periodontal disease, there were observed areas of more or less extended demineralization, erosion and necrosis. Our study showed that chronic periodontal disease may affect the tooth, as well, not only its support tissues.

Corresponding author: Laurentiu Mogoanta, Professor, MD, PhD; e-mail:

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Velimir Milosevic, Filip Vukmirovic, Miljan Zindovic, Miljan Krstic, Sanja Milenkovic, Snezana Jancic

Background: There is no information on the effects of leptin receptors expression on mucin-histochemical alterations in human colorectal adenocarcinoma. Aim: Testing the correlation of leptin receptors expression with histochemical dysregulation of mucins in colorectal adenocarcinoma. Patients and Methods: The study included 75 patients with colorectal adenocarcinoma who underwent surgical resection. Following a routine histopathological tissue analysis, 3-4 micro-m thick cuts were made onto resected tumors, which underwent a routine Hematoxylin-Eosin, histochemical Alcian Blue-Periodic Acid Schiff, pH 2.5, and High Iron Diamine-Alcian Blue, pH 2.5, methods for mucin differentiation and immunohistochemical Avidin-Biotin peroxidase complex method with anti-Ki67 and anti-leptin receptor antibodies. Following the quantification of results for the statistical analysis, the statistical software package SPSS for Windows (13.0) was used, and the tests for analyzing the significance of differences and correlation analysis - Spearman s rank correlation coefficient, were conducted. Results: Increased expression of leptin receptors is with highly significant correlation coefficient associated with hypersecretion of sialomucins. Significant positive correlation coefficient exists between the leptin receptors expression against neutral-fucomucins secretion. With weak and negative, but a significant correlation coefficient, leptin receptors expression is associated to the sulfomucins generation. Conclusions: Increased expression of leptin receptors in colorectal adenocarcinoma is associated with mucin-histochemical abnormalities that are manifested by sialomucins hypersecretion and reduction, ultimately resulting in the absence of sulfomucins secretion.

Corresponding author: Velimir Milosevic, MD, PhD; e-mail:

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