Intraocular biodistribution of intravitreal injected chitosan/gelatin nanoparticles

Vol. 55 No. 3 Suppl., 2014
This supplement was not sponsored by Outside Organizations.


Andreea Dana Moraru, Marcel Costuleanu, Anca Sava, Danut Costin, Catalina Peptu, Marcel Popa, Dorin Chiselita

The aim of this study was to evaluate intraocular biodistribution of a fluorescent polymeric nanosystem composed of chitosan and gelatin after intravitreal administration in rat eyes. The nanoparticles based on chitosan and gelatin were synthesized using a reverse emulsion-double cross-linking technique (ionic and covalent) and their structural characteristics are presented. Two units of 1% suspension of fluorescein-labeled nanoparticles in saline solution were injected intravitreal in rat eyes. The histological cross-sections obtained at 24 and 72 hours were analyzed by confocal microscopy and compared to a similar number of control cross-sections. The scanning electron microscopy of the nanoparticles obtained by double cross-linking in reverse emulsion technique revealed spherical, smooth, highly porous particles with no tendency to form aggregates. The chitosan-fluorescein conjugate was present in all the ocular tissues both at 24 and at 72 hours. The nanoparticles were present in the retina in a larger quantity and persisted longer than in the other ocular tissues. They were mainly fixed paravascular. The double cross-linking in reverse emulsion technique was efficient in synthesizing a biocompatible polymeric nanosystem. The in vivo study of intraocular biodistribution of fluorescein-labeled nanoparticles revealed their affinity for the retina after intravitreal administration.

Corresponding author: Anca Sava, MD; e-mail:

Download PDF


Anca Daniela Stanescu, Elena Nistor, Maria Sajin, Alex Emilian Stepan

Uterine myometrial smooth muscle tumors sometimes raise concerns related to their histopathological typing, and that especially for benign entities with areas of atypia. In this study, we analyzed a group of myometrial smooth muscle tumors in what it regards their differential diagnosis utilizing a panel of antibodies consisting of alpha-SMA, Ki-67, p16, p53, PR and bcl-2. The study included a total of 16 cases of cellular leiomyomas, five cases of atypical leiomyomas and six of leiomyosarcomas, which were all analyzed for age, risk factors, mitotic activity, cellular atypia and tumor staging. In cases of cellular leiomyomas, the immunophenotype was characterized by high expression of PR and bcl-2 and low expression of p53 and Ki-67. For atypical leiomyomas, PR, bcl-2 and p53 expression levels were highly compared with low levels of p16 and Ki-67. Leiomyosarcomas immunophenotype was characterized by high expression values of Ki-67, p16 and p53, and decreased levels of PR and bcl-2. The study indicated specific immunophenotypes among the uterine myometrial smooth muscle tumors analyzed. The antibody panel used here might be a useful for the complementary histopathological analysis of myometrial leiomyomas and leiomyosarcomas.

Corresponding author: Alex Emilian Stepan, University Assistant, MD, PhD; e-mail:

Download PDF
Download cover
Download contents

Journal archive