Isolation and characterization of chorionic mesenchyal stem cells from the placenta
Vol. 52 No. 3 Suppl., 2011
This supplement was not sponsored by Outside Organizations.
ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY
D. Rus Ciuca, Olga Soritau, S. Susman, V. I. Pop, Carmen Mihaela Mihu
The aim of the study was the isolation and characterization of mesenchymal stem cells from the placental chorion from a genotypical and phenotypical point of view. The placentas included in the study were derived from term pregnancies with a normal evolution. Along with the placentas, umbilical cord blood, maternal and newborn peripheral blood samples were taken. The isolation and culture of chorionic and, incidentally, trophoblastic cells was followed by the determination of markers of the former cells. They expressed proteins and genes characteristic of stem cells. Immunofluorescence and evaluation of gene expression evidenced the pluripotential properties of these cells and also their higher position on the differentiation pathway. HLA expression provides information that might help explain the immunological mechanisms of tolerance between the maternal organism and fetal structures.
Corresponding author: Carmen Mihaela Mihu, Associate Professor, MD, PhD, e-mail: carmenmihu2004@yahoo.com
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Florina Carmen Popescu, Cristina Jana Busuioc, G. D. Mogosanu, O. T. Pop, H. Parvanescu, I. Lascar, Corina Ivona Nicolae, L. Mogoanta
Pericytes and myofibroblasts are two types of connective cells that appear in large quantities in reparative processes. In order to evaluate their response and any relationships between them, we have inflicted third degree skin burns to seven groups of five common Wistar rats each from which we sampled granulation tissue at three days interval for histological and immunohistochemical studies. Alpha-SMA and CD34 antibodies revealed that granulation tissue myofibroblasts do not express CD34 and do not arise from pericytes. The parallel arrangement of myofibroblasts in relation to the burned wound surface suggests that their main function is mechanical traction on granulation tissue and wound edges.
Corresponding author: Laurentiu Mogoanta, Professor, MD, PhD, e-mail: laurentiu_mogoanta@yahoo.com
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