Enhanced dendritic arborization of hippocampal CA3 neurons by Bacopa monniera extract treatment in adult rats
Vol. 52 No. 3 Suppl., 2011
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ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY
Venkata Ramana Vollala, Subramanya Upadhya, Satheesha Nayak
Objective: Bacopa monniera (BM), a traditional Ayurvedic medicine has been used in treatment for a number of disorders, particularly those involving anxiety, intellect and poor memory. The current study examined the effects of standardized extract of Bacopa monniera on the dendritic morphology in adult rats of hippocampal CA3 neurons, one of the regions concerned with learning and memory. Materials and Methods: Adult Wistar (2.5-month-old) rats were designated into 2-, 4- and 6-week treatment groups. Rats in each of these groups were divided into 20 mg/kg, 40 mg/kg and 80 mg/kg dose groups (n=8 for each dose). These rats along with age-matched control rats were then subjected to spatial learning (T-maze) and passive avoidance tests. Subsequent to the T-maze and passive avoidance tests, these rats were killed by decapitation, brains were removed and hippocampal neurons were impregnated with silver nitrate (Golgi staining). Hippocampal CA3 neurons were traced using camera lucida. Dendritic branching points (a measure of dendritic arborization) and dendritic intersections (a measure of dendritic length) were quantified. These data were compared with control rats. Results and Conclusions: The results showed improvement in spatial learning performance and enhanced memory retention in rats treated with BM extract. There was a significant increase in the dendritic intersections and dendritic branching points along the length of both apical and basal dendrites in rats treated with BM extract for four and six weeks. However, the rats treated with BM extract for two weeks did not show any significant change in hippocampal CA3 neuronal dendritic arborization. We conclude that constituents present in BM extract have neuronal dendritic growth stimulating properties.
Corresponding author: Venkata Ramana Vollala, Assistant Professor, MSc, PhD, e-mail: ramana.anat@gmail.com
Download PDF Enhanced dendritic arborization of hippocampal CA3 neurons by Bacopa monniera extract treatment in adult rats PDFROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY
Adina Octavia Duse, Raluca Amalia Ceausu, T. Mezei, Anca Maria Cimpean, Pusa Gaje, Hortensia Ionita, I. Jung
Only few data are available in the literature concerning angiogenesis in hematological malignancies. Non-Hodgkin lymphoma classified on the base of molecular profile is frequently characterized by unpredictable behavior that seems to be related to tumor cells and also to the tumor microenvironment. The tumor microenvironment contains blood vessels and a large variety of cells that can play an important role to the progression of angiogenesis and tumor growth. From these, mast cells have been shown to be a source of angiogenic factors. The aim of this work was to investigated the relationships between mast cells and blood vessels in non-Hodgkin lymphoma and reactive lymphoid tissue from three different anatomical sites. Using double immunostaining method CD34/mast cell tryptase we noticed that mast cell density was significantly lower in the follicular lymphoma than in diffuse type lymphoma. The morphology of vessels, the presence of pillars and splitting suggested that intussusceptions is the main mechanism of angiogenesis. In the cases with primary lymphoma of the spleen, we found few mast cells and a high number of blood vessels. Our data suggest that lymphoma-associated angiogenesis is driven in part by the tumor microenvironment, and particularly, by mast cells. On the other hand, our results support the organ-specific tumor-associated angiogenesis in malignant non-Hodgkin lymphoma.
Corresponding author: Anca Maria Cimpean, Associate Professor, MD, PhD, e-mail: ancacimpean1972@yahoo.com
Download PDF Mast cells contribute to the angiogenesis in non-Hodgkin lymphoma. An immunohistochemical study based on the relationship with microvessel density PDF
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