The genotoxicity study of resveratrol in primary gastric adenocarcinoma cell cultures

Vol. 50 No. 3, 2009

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

P. Mitrut, Fl. Burada, Aurelia Enescu, R. Scorei, Daniela Badea, Amelia Genunche-Dumitrescu, I. Rogoz, Madalina Manea

Gastric cancer is the second most common cause of cancer-related death in the world. Some studies indicate that polyphenolic compounds and antioxidants exert a protective action against gastric cancer. Among the polyphenolic compounds tested and proven effective against gastric cancer is resveratrol, a natural polyphenol present in red wines and various human food items. Resveratrol has been shown to suppress proliferation of a wide variety of tumor cells. We tested the genotoxic activity of resveratrol in primary cell cultures from gastric adenocarcinoma, obtained by mucosal biopsy at upper digestive endoscopy. The adenocarcinoma cells were analyzed for the presence of micronuclei at different concentrations of resveratrol at 48 hours and at 72 hours. The results showed that resveratrol induced micronuclei dose-dependently. The frequency of micronuclei increased progressively with the dose of resveratrol used, the high frequency is in the primary culture initiated from gastric adenocarcinoma: signet ring cell type. The high frequency of micronuclei is at 72 hours at the 20 micro-g/mL resveratrol and is decreased at low concentrations (5 micro-g/mL, 10 micro-g/mL resveratrol). This results show the genotoxic activity of resveratrol in adenocarcinoma gastric cell and the anticancer property of this substance.

Corresponding author: Paul Mitrut, MD, PhD, e-mail: centrul_renasterea@rdscv.ro

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