Protective role of alpha lipoic acid against polychlorobiphenyl (Aroclor 1254)-induced testicular toxicity in rats

Vol. 57 No. 2 Suppl., 2016
This supplement was not sponsored by Outside Organizations.

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Ozay Gules, Ulker Eren

The present study was aimed to investigate the antioxidant, biochemical, and histological effects of alpha lipoic acid (ALA) on polychlorinated biphenyl (PCB)-induced testicular toxicity in male rats. The rats were divided into five groups: In the control group, the rats were not administered any chemicals for 30 days. In the sham group, the rats were administered corn oil for 30 days. In the PCB group, the rats were administered with Aroclor 1254 for 30 days. In the ALA group, the rats were treated with ALA for 30 days. In the ALA+PCB group, the rats were treated with ALA 24 hours before Aroclor 1254 was administered for 30 days. The total oxidant status (TOS) level in the serum and testis, number of apoptotic cells, vacuolization at the basal membrane, immature spermatids in the tubular lumen, heme oxygenase-1 (HO-1) staining density, and abnormal spermatozoa were significantly increased in the PCB group. Moreover, in the PCB group, the seminiferous tubule diameter (STD) was decreased in stage VII-VIII and XII-XIV tubules. The TOS level in the serum and testis, vacuolization at the basal membrane, immature spermatids in the tubular lumen, and apoptosis were significantly decreased in the ALA+PCB groups. These findings suggested that ALA has a protective role against PCB-induced testicular toxicity.

Corresponding author: Ozay Gules, Assistant Professor; e-mail: ogules35@gmail.com

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ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Ionela Teodora Dascalu, Horia Octavian Manolea, Evantia Coles, Constantin Daguci, Marilena Bataiosu, Cristina Maria Andrei, Mihaela Jana Tuculina, Oana Andreea Diaconu, Luminita Daguci

The research aims at emphasizing the interrelation between the social impact and the self-esteem of schoolchildren with dental crown fractures in the frontal group. Dental traumatic injuries are frequently met to schoolchildren, therefore, the prevention and interception of traumatic pathology within parents, teachers and children is more than necessary. Our statistical study intends to determine the prevalence of dental trauma to southwestern Romanian schoolchildren aged 6 to 14. The survey included a number of 1684 schoolchildren attending public schools, randomly chosen. Data were collected from October 2012 to December 2013. Clinical examination was conducted to assess the dental traumatic injuries. Age and gender distribution, etiological factors, risk factors and the cause of injuries were the parameters taken into consideration. The data collected was processed and analyzed using the SPSS (Statistical Package for the Social Sciences) statistical software. The overall prevalence of dental trauma was 4.63% amongst the subjects examined. The maxillary central incisor was the most commonly affected tooth (85.19% in boys and 97.87% in girls). We observed a significant statistical difference in the number of children with one, two or three fractured teeth from the urban and rural areas. The percentage of schoolchildren with crown fractures that we examined was of 3.09% in the urban area and 1.54% in the rural area. The traumatic injuries of the maxillary upper teeth were most prevalent. Since the dental trauma of incisors has a strong impact on the quality of life of children and their families, health preventive policies are needed in order to avoid psychological implications on the lifelong existence of children affected.

Corresponding author: Constantin Daguci, DMD, PhD; e-mail: dagucicristi@yahoo.com

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