Local cause of gingival overgrowth. Clinical and histological study

Vol. 57 No. 2 Suppl., 2016
This supplement was not sponsored by Outside Organizations.


Emma Cristina Draghici, Stefania Craitoiu, Veronica Mercut, Monica Scrieciu, Sanda Mihaela Popescu, Oana Andreea Diaconu, Bogdan Oprea, Roxana Maria Pascu, Monica Mihaela Craitoiu

The overgrowth, depending on its extension, has multiple effects on the stomatognathic apparatus: functional disorders (impaired speech), difficulty in chewing and aesthetic problems but can cause significant psychological problems. We proposed this study, motivated by the relative increased frequency of the gum outgrowth, its multifactorial etiopathogeny, but especially from the point of view of the specialist practitioner, by the problems that this pathology raises not only for the functionality of the stomatognathic apparatus but also for the facial esthetics, and especially for future therapeutic attitudes needed to solve the existing pathology at this level. We conducted a clinical study and a histological one. For the clinical study, we selected 74 patients who experienced different degrees of gingival outgrowing associated with fillings, dental caries, fixed prostheses, mobile prostheses, orthodontic apparatus. Thirty gingival fragments from patients with gingival outgrowing were processed by paraffin-embedding histological technique and stained with Hematoxylin-Eosin. The morphological results obtained provide the necessary support for understanding the possibility of developing a therapeutic strategy to prevent or minimize the gum outgrowth by administering antibiotic and anti-inflammatory medications associated with medications, which shall cause the apoptosis of the fibroblasts.

Corresponding author: Stefania Craitoiu, Professor, MD, PhD; e-mail: scraitoiu@yahoo.com

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Mustafa Yildiz, Mustafa Sandikci

Free radicals increase in the presence of diabetes. Lycopene is a powerful antioxidant. The goal of the present study was to determine the effect of diabetes on rat ovaries and the protective role of lycopene in that context. Experimental diabetes was induced with 50 mg/kg streptozotocin. Rats were randomly separated into four groups, as follows: control + corn oil, control + lycopene, diabetes + corn oil and diabetes + lycopene. The histological and histometric evaluations were performed using Crossmans triple staining method. The immuno-histochemical connexin-43 expression was identified and the apoptotic cell density was determined using the terminal deoxynucleotidyl transferase dUTP nick-end labeling method, while the malondialdehyde levels were measured using the enzyme-linked immunosorbent assay technique in the ovaries. Vacuolization of the corpus luteum, hydropic degeneration in the interstitial regions, and the number of corpora lutea increased in the ovary as effects of diabetes while the diameter of the corpora lutea decreased. The intensity of connexin-43 expression decreased in the primordial and atretic follicles, interstitial cells and luteal cells of the corpora lutea in the diabetes + corn oil group. The ovarian malondialdehyde levels and the number of apoptotic cells in the granulose layers of the large antral follicles increased in the presence of diabetes. Lycopene increased the expression of connexin-43 in the primordial, secondary and large antral follicles in the ovaries of diabetic animals. The changes caused by diabetes in the ovaries and the protective role of lycopene in some but not all parameters was revealed.

Corresponding author: Mustafa Yildiz; e-mail: mustafayildiz17@yahoo.com

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