Prognostic significance of MMP-9 and TIMP-1 in liver metastases

Vol. 56 No. 2 Suppl., 2015
This supplement was not sponsored by Outside Organizations.


Simona Eliza Giusca, Irina-Draga Caruntu, Cornelia Amalinei, Elena-Roxana Avadanei

Our research focuses both on the correlations between MMP-9 and TIMP-1 and classical clinicopathological factors and on the prognostic value of MMP-9 and TIMP-1 for survival. The study group included 52 patients diagnosed with hepatic metastases. The tissue specimens have been specifically processed for immunohistochemical exam, by using anti-MMP-9 and anti-TIMP-1 antibodies. For the semi-quantitative assessment, we have used an individualized score, which values allowed the discrimination of two classes of cases (low and high), using two different thresholds: <=4 and <4. Data have been statistically analyzed by using Fisher 2x2 test and Kaplan-Meier curves. Statistical analysis between MMP-9 and TIMP-1 expression (low versus high, separately for each threshold) and clinicopathological characteristics had not revealed significant differences. In both types of threshold applied in survival analysis, significant differences between MMP-9 and TIMP-1 low and high expression have been demonstrated. For cases with concordant MMP-9-TIMP1 co-expression, low versus high, the survival analysis revealed that threshold value <4 offers a better stratification of cases when compared to threshold value <=4, based on significant differences registered only for threshold value <4. No significant differences were registered between cases with discordant MMP-9-TIMP-1 co-expression, for both thresholds. Regardless of the used threshold, the survival analysis achieved between the cases with MMP-9-TIMP-1 concordant co-expression and cases with MMP-9-TIMP-1 discordant co-expression had proven significant differences. Our study suggests that the confirmation of MMP-9 and TIMP-1 value as prognostic factors, based on immunohistochemical expression, requires a threshold validation.

Corresponding author: Irina-Draga Caruntu, Professor, MD, PhD; e-mail:

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Roxana-Ioana Nedelcu, Sabina Andrada Zurac, Alice Brinzea, Mirela-Daniela Cioplea, Gabriela Turcu, Raluca Popescu, Catalin-Mihai Popescu, Daniela Adriana Ion

Halo (Sutton s) phenomenon has been described as a depigmented halo that is associated most commonly with acquired melanocytic nevi; but it may be associated with various types of melanocytic skin tumors, melanoma being the most concerning. Different authors have been preoccupied with elucidating morphological features of melanocytic tumors associated with a depigmented halo. We reviewed the literature and discussed the main features of melanocytic halo tumors regarding histopathological, immune microenvironment profile and dermatoscopic appearance. We highlighted similarities and differences between Sutton s nevus and halo melanoma, also presenting relevant aspects of our results. Depigmented halo must be regarded as a phenomenon that may be associated with different types of melanocytic tumors and with a broad spectrum of histopathological atypia degree. Certain correlations between the shape, diameter, symmetry observed in clinical examination, histopathological appearance, dermatoscopic aspect of peritumoral halo and central tumor type could not be established due to insufficient data and contrasting results. Further studies are expected to add valuable information regarding the depigmented halo tumors features.

Corresponding author: Roxana Ioana Nedelcu, MD, PhD; e-mail:

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