Influence of Staphylococcus aureus attachment to the herpes simplex virus infected cells

Vol. 56 No. 2 Suppl., 2015
This supplement was not sponsored by Outside Organizations.


Coralia Bleotu, Mariana Carmen Chifiriuc, Veronica Lazar, Roxana Dragusel, Lilia Matei, Ioana Madalina Aldea, Laura Denisa Dragu, Bogdan Ionescu, Carmen Cristina Diaconu, Costin Cernescu, Carmen Aurelia Mogoanta, Cristian Radu Popescu, Bogdan Popescu, Raluca Grigore, Serban Vifor Gabriel Bertesteanu

The purpose of this study was to investigate the response of HeLa cells to the interaction with inactivated Staphylococcus aureus cells and live challenge with herpes simplex virus (HSV).The results of this study are indicating that the interaction between the HeLa cells and S. aureus inactivated whole cells could modulate the host cell apoptosis and cytokine production, and therefore, influence the progression of HSV infection. The pre-treatment of HeLa cells with heat inactivated bacterial whole cells protects them from the occurrence of HSV mediated cytopathic effect, while the post viral infection treatment with bacterial cells prevents the high activation of bax/bcl-2 apoptotic pathway, a process that could change the fate of the infectious process triggered by the virus, and eventually reduce its multiplication rate. The pre-treatment of HeLa monolayer with inactivated bacterial cells 24 hours before the viral infection is increasing the expression level of TNF-alpha, IL-6 and IL-8 pro-inflammatory cytokines genes, also suggesting that bacterial antigens could contribute to the decrease of viral multiplication rate.

Corresponding author: Raluca Grigore, Lecturer, MD, PhD;

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Remus Sebastian Sipos, Radu Fechete, Ramona Ioana Chelcea, Dumitrita Moldovan, Zsuzsanna Pap, Zoltan Pavai, Dan Eugen Demco

The effects of two wonder drugs, simvastatins and fenofibrates on the proximal part of the femoris of a series of ovariectomized and non-ovariectomized Wistar albino rats was estimated qualitatively and semi-quantitatively by the modern method of 1D 1H-NMR T2-distribution. The 72 rats subjected to this study were divided in six groups and were sacrificed at two, four, six and eight weeks after ovariectomy and the proximal part of femoris was harvested. The CPMG (Carr-Purcell-Meiboom-Gill) echoes train curves were measured for the bones fully saturated with water during two months after two months of natural drying. These decays were analyzed by Laplace inversion and an average of normalized T2-distributions was considered for all rat s groups. The 1D averaged T2-distributions present four peaks, which were associated with protons in four major environments, from which the free water protons are used as spy molecules to explore the boundaries of cavities. In the approximation of spherical pores, the averaged T2-distributions were transformed in distributions of pores diameters. These were found in the range from 2 micro-m up to 2 mm. The relative amplitudes, widths and position of deconvoluted distributions of small, medium and large cavities are used for a qualitatively analysis of the effect of our lipid-lowering drugs. For a semi-quantitatively analysis, we chose the diameter d of proximal part of femoris trabecular cavities. We show that the positive or negative effects of treatments with simvastatins and fenofibrates are strongly dependent on the duration of treatment. Moreover, the treatment of healthy bone is generally counter-indicated.

Corresponding author: Remus Sebastian Sipos, Assistant Professor, e-mail:; Radu Fechete, Associate Professor, Dr. Habil., e-mail:

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