An animal model of peripheral nerve regeneration after the application of a collagen-polyvinyl alcohol scaffold and mesenchymal stem cells

Vol. 55 No. 3 Suppl., 2014
This supplement was not sponsored by Outside Organizations.


Silviu-Adrian Marinescu, Otilia Zarnescu, Ioana-Ruxandra Mihai, Carmen Giuglea, Ruxandra Diana Sinescu

Extensive nerve injuries often leading to nerve gaps can benefit, besides the gold standard represented by autologous nerve grafts, by the inciting field of tissue engineering. To enhance the role of biomaterials in nerve regeneration, the nerve conduits are associated with Schwann or Schwann-like cells. In this study, we evaluated rat sciatic nerve regeneration, by using a biodegradable nerve guide composed of Collagen (COL) and Polyvinyl Alcohol (PVA), associated with mesenchymal stem cells (MSC). After the exposure of the rat sciatic nerve, a nerve gap was created by excising 1 cm of the nerve. Three experimental groups were used for nerve gap bridging: autografts, nerve conduits filled with medium culture and nerve conduits filled with MSC. The methods of sensory and motor assessment consisted of the functional evaluation of sciatic nerve recovery - toe-spread, pinprick tests and gastrocnemius muscle index (GMI). The histological and immunocytochemical analysis of the probes that were harvested from the repair site was performed at 12 weeks. Successful nerve regeneration was noted in all three groups at the end of the 12th week. The functional and immunocytochemical results suggested that COL-PVA tubes supported with mesenchymal stem cells could be considered similar to autologous nerve grafts in peripheral nerve regeneration, without the drawbacks of the last ones. The functional results were better for the autografts and the ultrastructural data were better for the nerve conduits, but there were not noticed any statistical differences.

Corresponding author: Ioana-Ruxandra Mihai, Scientific Researcher, MD, PhD; e-mail:

Download PDF


Anca Stefania Enescu, Aurelia Enescu, Maria Balasoiu, Mircea-Sorin Ciolofan, Alina-Nicoleta Capitanescu

The pleomorphic adenoma, also known as mixed tumor, presents an epithelial and mesenchymal histological structure, being also called epithelioma with altered stroma. The pleomorphic adenoma has a frequency of 70% of major salivary gland tumors, being located preponderantly at the level of parotid gland (52-84%), at the level of submandibular glands (7-17%) and at the level of the other salivary minor salivary glands (3-8%). The purpose of our study was the histopathological analysis of 45 cases of pleomorphic adenoma. The tumors were characterized by an increased structural pleomorphism, given by the multitude of cytological differences and proliferation patterns, and on the other side by the diversity of stromal component._x000D_

Corresponding author: Aurelia Enescu, Associate Professor, MD, PhD; e-mail:

Download PDF
Download cover
Download contents

Journal archive