Iron oxide nanoparticles modulate the interaction of different antibiotics with cellular membranes

Vol. 55 No. 3 Suppl., 2014
This supplement was not sponsored by Outside Organizations.

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Claudia Mihaela Istrate, Alina Maria Holban, Alexandru Mihai Grumezescu, Laurentiu Mogoanta, George Dan Mogosanu, Tudor Savopol, Mihaela Moisescu, Minodora Iordache, Bogdan Stefan Vasile, Eugenia Kovacs

The interaction of nanomaterials with cells and lipid bilayers is critical in many applications such as phototherapy, imaging and drug/gene delivery. These applications require a firm control over nanoparticle-cell interactions, which are mainly dictated by surface properties of the nanoparticles. The aim of this study was to investigate the interaction of Fe3O4 nanoparticles functionalized with several wide use antibiotics with opossum kidney (OK) cellular membranes in order to reveal changes in the membrane organization at different temperatures. We also investigated the in vivo biodistribution of the tested nanoparticles in a mouse model. Our results showed that, at low temperatures (31-35 degrees C), plain Fe3O4 nanoparticles induced a drop of the membrane fluidity, while at physiological or higher temperatures (37-39 degrees C) the membrane fluidity was increased. On the other hand, when nanoparticles functionalized with the tested antibiotics were used, we observed that the effect was opposite as compared to control Fe3O4 nanoparticles. Although most of antibiotics, used as plain solutions or linked on magnetite nanoparticles, proved heterogeneous effect on in vitro OK cells membrane fluidity, the aminoglycosides streptomycin and neomycin, used both as plain solutions and also combined with nanoparticles kept the same effect in all experimental conditions, increasing the membrane fluidity of OK cells plasma membrane. In vivo results showed that the antibiotic functionalized nanoparticles have a similar biodistribution pattern within the mouse body, being transported through the blood flow and entering the macrophages through endocytosis. Functionalized magnetite nanoparticles manifested a preferential biodistribution pattern, clustering within the lungs and spleen of treated mice. These results demonstrate that antibiotics manifest a different effect on plasma membrane fluidity depending on their type and temperature. Magnetite nanoparticles may interfere with antibiotic-cellular interactions by changing the plasma membrane fluidity. The fact that the antibiotic functionalized magnetite nanoparticles have a similar biodistribution pattern, are transported through the blood flow, and they increase the cellular uptake of the drug, suggest that they may be used for further studies aiming to develop personalized targeted delivery and controlled release nanoshuttles for treating localized and systemic infections.

Corresponding author: Alexandru Mihai Grumezescu, Chem. Eng., PhD; e-mail: grumezescu@yahoo.com

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ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Oana Cristina Mirea, Anca Adriana Ancuta, Mircea Sebastian Serbanescu, Paraschiva Postolache, Constantin Daniel Uscatu, Catalina Marin, Iancu Emil Plesea, Luminita Cristina Chiutu

Aortic size is known to be a predictor for cardiovascular deaths. The purpose of this study was to investigate whether aortic diameters measured on tissue samples obtained during autopsy from subjects that died of cardiovascular disease were bigger when compared to those from subjects deceased from other cause than cardiovascular disease. The study included 91 deceased subjects (average age 56+/-18.1 years), which underwent autopsy to determine cause of death. Morphological measurements were completed on 364 aortic specimens obtained from four different sites, namely ascending aorta, aortic arch, distal thoracic aorta and abdominal aorta. Aorta showed the tendency to decrease in diameter from ascending aorta to abdominal aorta, the latter presenting with the smallest diameter. All studied aortic diameters were found to be larger in the cardiovascular population (p<0.01).

Corresponding author: Iancu Emil Plesea, Professor, MD, PhD; e-mail: pie1956@yahoo.com

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