Short-reactivation of Neurogenin-3 and mesenchymal microenvironment is require for beta-cells differentiation during fetal pancreas development and islet regeneration

Vol. 55 No. 2 Suppl., 2014
This supplement was not sponsored by Outside Organizations.


Kaiming Yang, Yong Wang, Zhaokang Du, Xiujun Zhang

Purpose: To investigate influencing factors of beta-cells differentiation and microenvironment in embryonic development and regeneration, in order to conduct therapeutic efforts to broaden beta-cells mass in diabetes. Materials and Methods: The expression of Ngn3 (Neurogenin-3) and microenvironment of beta-cells differentiation during embryonic pancreas development at 4-12 weeks of gestation and regeneration after pancreatic islet injure observed by immunohistochemical staining. Results: The results showed that the expression of Ngn3 not only in pancreas development but also in beta-cells regeneration in rat diabetic model (DM) by streptozotocin (STZ) treatment. Pancreatic mesenchymal tissue always accompanied by islet cells differentiation and there is a short expression of Ngn3 occurrence before all islet cell types differentiated from pancreatic epithelium. The expression of Ngn3 including ectopic expression also appearance in beta-cells injured rat pancreas. In addition, there are some Nestin-positive cells where located in pancreatic duct, islets and mesenchyme were detected in DM. Double immunostaining witness Brdu/Ngn3-positive cells was only in pancreatic mesenchyme after beta-cells injure. Conclusions: Our data demonstrated the expression of transcription factor Ngn3 and pancreatic mesenchymal microenvironment are important and necessary to promote pancreatic progenitors differentiated to islet cells regardless of pancreatic development or islets regeneration.

Corresponding author: Kaiming Yang, Professor, MD, PhD; e-mail:,

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Maria Balasoiu, Andrei Theodor Balasoiu, Stelian Stefanita Mogoanta, Alexandru Barbalan, Alex Emilian Stepan, Raluca Niculina Ciurea, Dragos Ovidiu Alexandru, Aurelia Enescu, Laurentiu Mogoanta

Introduction: Colorectal cancer represents a major cause of mortality and morbidity and occupies the third place as cancer incidence and the fourth place as cancer mortality. Colorectal cancer causes 608 000 deaths per year, despite correct applied treatment (surgery and chemotherapy). Interleukin 8 (IL-8) is an important proinflammatory cytokine with an important role in leukocyte chemoattraction. IL-8 also represents an important tumorigenic and proangiogenic factor. Materials and Methods: We have studied 68 patients aged between 55 and 70 years, hospitalized in the IInd Surgery Clinic of the Emergency County Hospital of Craiova, Romania. According to TNM grading, the patients were in stages II, III and IV. From these patients, we have prelevated two types of samples: serum and tumor fragment. Results: IL-8 values were significantly increased, both in serum and tumor supernatant. The highest IL-8 values were found in tumor supernatant and in advanced stages; IL-8 increased values were correlated with tumor growth and TNM stage. Conclusions: IL-8 has an important role in colorectal cancer growth and metastasis and represents an important therapeutic target in this type of cancer. IL-8 represents also a predictor for colorectal cancer prognosis.

Corresponding author: Andrei Theodor Balasoiu, MD, PhD student; e-mail:

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