Precursor and interstitial Cajal cells in the human embryo liver

Vol. 55 No. 2 Suppl., 2014
This supplement was not sponsored by Outside Organizations.


Mugurel Constantin Rusu, Irina Duta, Andreea Cristiana Didilescu, Alexandra Diana Vrapciu, Sorin Hostiuc, Emil Anton

Interstitial Cajal Cells (ICCs) were only proven in human adult hepatic tissue. The immune phenotypes of various cell types in the human embryonic liver (HEL) are scarcely described. It was hypothesized that in HEL ICCs are present and distinctive to the precursor/progenitor cells populations. It was aimed and performed a qualitative study of HEL by use of antibodies against CD117/c-kit, CD31, CD34, CD90, CD105, DOG1, Ki67, and adiponectin. Five human embryos of 23-29 mm were used. Blasts and hematopoietic cells were comprising the two major cell populations in late stage embryos. The general population of blasts in the HEL was CD34-/CD105, although scarce CD117/c-kit+ and CD90+ such cells were found. Hematopoietic precursors were Ki67+. Adiponectin-positive plasmalemmas were found mostly in blasts. Endothelia were CD31+/CD34+. Interstitial cells with moniliform prolongations were found; such cells were scarcely CD117/c-kit+ but consistently DOG1+. They were diagnosed as ICCs but based on the morphology of their prolongations they can be equally viewed as being telocytes (TCs). Further studies should better correlate the precursor cell-types and immune phenotypes during human liver organogenesis. Liver ICCs and/or TCs should be also investigated in the human fetal liver.

Corresponding author: Mugurel Constantin Rusu, Associate Professor, MD, PhD, Dr. Hab.; e-mail:

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Mihai Raul Popescu, Gigi Calin, Irina Strambu, Marian Olaru, Maria Balasoiu, Veronica Huplea, Catalina Zdrancota, Razvan Mihail Plesea, Stelian Danut Enache, Iancu Emil Plesea

Aim: The authors assessed the mycobacterial lesions in the lymph nodes discovered on tissue samples coming from the surgical stage in the Department of Pathology, Emergency County Hospital of Craiova, Romania, starting from 1990 until 2012. Materials and Methods: The studied material consisted of lymph node (LN) tissue samples obtained by biopsy or surgical excision from 362 patients histopathologically diagnosed with tuberculosis. For confirming the diagnosis, Ziehl-Neelsen staining was carried out and, in some cases, PCR technique was used. Results: Patients were mainly women, with a mean age of 35 years. The suspicion of diagnosis at admission was reduced, the most usual diagnosis being a very general and uncertain one of adenopathy/polyadenopathy. In only few cases, other tissues/organs have been affected in the same time with the LN determination. Also, multiple LN group involvement was present in only five cases. The most affected LN groups were: the lateral cervical, submandibular, axillary, inguinal, supraclavicular and mesenteric. In paired LN groups, there was no predilection for any of the body sides. Epithelioid cells (ECs) and giant Langhans cells (GLCs) were present together in most of the granulomatous reactions. However, the presence of neutrophils in 10% of the cases should be noticed. Necrosis was present in almost all cases, displaying the whole range of morphological features, but usually the acidophilic, microgranular one. Fibrosis was rarely encountered. As a whole, well-differentiated granulomas were the most frequent but the presence of hyporeactive granulomas in more than a quarter of the cases and that of non-reactive granulomas in more than 10% of the cases should be noticed. The extension of TB process was not a rare event. Conclusions: LNs seem to be the favorite location of TB besides the lung. The overall morphological picture revealed an active and destructive profile of the bacillary aggression in the LN parenchyma, which could mean either a higher sensitivity of the LN tissue or a more vulnerable background of the patients with TBLN lesions.

Corresponding author: Iancu Emil Plesea, Professor, MD, PhD; e-mail:

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