Immunohistochemistry of a choroidal melanoma: nestin, CD34 and CD117/c-kit labeling

Vol. 55 No. 2 Suppl., 2014
This supplement was not sponsored by Outside Organizations.


Alexandra Diana Vrapciu, Mugurel Constantin Rusu, Liliana Mary Voinea

In a case of choroidal melanoma (CM) in a 70-year-old male patient, was firstly aimed at studying the processes of angiogenesis by use of nestin and CD34 antibodies. Anti-CD117/c-kit antibodies were further considered for their progenitor cells specificity. Choroidal melanoma was histopathologically confirmed. Nestin-positive endothelia were found in the CM and the adjacent retina, but not in endothelia elsewhere in that eye. Nestin-positive non-pigmentary cells were found within the CM. Filopodia-projecting endothelial tip cells (ETCs), nestin- and CD34-positive were found in the CM. CD34-positive ETCs were also found in the iridial stroma. There were found two different immune patterns of the retinal Muller cells (MCs). They were nestin-positive in the retina adjacent to the tumor, but negative in any other part of retina. On the other hand, CD117/c-kit antibodies labeled MCs as follows: (a) discontinuously, or continuously, in the retina adjacent to the CM; (b) only the inner segments of the MCs were labeled in the retina unrelated to the CM. While nestin could be a reliable marker for retinal damage, the CD117/c-kit phenotype of MCs still needs further investigations. Antiangiogenic therapy appears as a good choice for tumor therapy.

Corresponding author: Mugurel Constantin Rusu, Associate Professor, MD, PhD, Dr. Hab.; e-mail:

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Camelia Ionescu, Bogdan Mihai Galbinasu, Horia Manolea, Ion Patrascu

Clinical studies have revealed that the main objective of implants in the edentulous jaw is to provide support for fixed prostheses or to stabilize complete dentures. Various attachment systems were developed for universal use in partially and completely edentulous patients such as clasps, cone-shape telescope copings, magnets, bar systems, locators. The aim of this case report is to present the Locator attachment that does not use the splinting of implants. Four implants were placed in the foraminal region and the Locator attachment system was used to connect overdentures to mandibular dental implants. The results proved that the Locator attachment system offers the possibility to obtain a higher retention and an improved stability for overdentures in edentulous patients with a severely resorbed mandible and lack of vertical space between the arches.

Corresponding author: Bogdan Mihai Galbinasu, Assistant, MD, PhD; e-mail:

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