Immunohistochemical expression of RBP2 and LSD1 in papillary thyroid carcinoma

Vol. 54 No. 3 Suppl., 2013
This supplement was not sponsored by Outside Organizations.

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Lingling Kong, Guoan Zhang, Xu Wang, Jian Zhou, Sen Hou, Wen Cui

Purpose: To investigate the prognostic significance of LSD1 and RBP2 expression in patients with papillary thyroid carcinoma. Materials and Methods: LSD1 and RBP2 expressions were detected by immunohistochemistry in surgically resected samples from thyroid adenoma, papillary thyroid carcinoma and paracancerous tissues. Results: To be members of histone demethylases, LSD1 and RBP2 were both localized mainly to the thyroid cell nucleus. Despite the fact that both RBP2 and LSD1 expressions were higher in papillary thyroid carcinoma than in paracancerous tissues (U=-3.855, p=0.000; U=-5.575, p=0.000) and thyroid adenoma (U=-1.972, p=0.049; U=-3.190, p=0.001), they did not show us statistical correlation (r=-0.149, p=0.270). Like LSD1 (U=-2.286, p=0.022), RBP2 expression was less frequently in paracancerous tissues than in thyroid adenoma (U=-1.985, p=0.047). Neither LSD1 nor RBP2 expression was significantly associated with age, gender, stage status, tumor size, and lymph node metastases (p>0.05). Conclusions: Both LSD1 and RBP2 are well related with the occurrence and malignant transformation of papillary thyroid carcinoma. Though the positive expression of both LSD1 and RBP2 can be used to estimate the potentiality of thyroid carcinoma and help for the adjuvant treatment, LSD1 is a more sensitive molecular marker than RBP2 on thyroid cancer diagnosis.

Corresponding author: Lingling Kong, Associate Professor; e-mail: Kong001199@yahoo.com; Wen Cui, Professor; e-mail: cuiwenmd@163.com

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ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Catalina Poiana, M. C. Neamtu, Elena Taina Avramescu, Mara Carsote, Raluca Trifanescu, Dana Terzea, Oana Maria Neamtu, D. Ferechide, Rucsandra Danciulescu Miulescu

Background: The G2 neuroendocrine tumors (NET) or well-differentiated neuroendocrine carcinomas (2010 WHO Classification of Tumours of the Digestive System) embrace different types of evolution despite the fact that they actually are included in the same group of prognosis based on mitotic count and the Ki-67 proliferation index. Aim: We studied the pathological and clinical aspects in patients with G2 NET. Materials and Methods: This is a retrospective pilot observational study in patients admitted between January 2008 and January 2013 in "Constantin I. Parhon" National Institute of Endocrinology, Bucharest, Romania. They were evaluated based on the pathological report, imagistic scan, and neuroendocrine markers. Results: Nine patients (female/male ratio: 5/4) with G2 NET were included (mean age at diagnosis 54.11 years). Surgery was performed in 66.66% of cases. 44.44% of tumors had unknown origin. 22.22% of patients had negative immunostain for chromogranin A. Synaptophysin was positive in all cases. Neuronal specific enolase (NSE) was performed in 44.44% of cases and it was positive in all these situations. 88.88% of patients had high neuroendocrine markers. Multiple tumors were found in two cases (follicular thyroid adenoma, and a carcinoma of the port vein, respective bilaterally pheochromocytomas). The youngest patient (39-year-old) had atypical onset with bilateral adrenal tumors (positive for CHROMO, EMA, CK-19, CK-20, negative for SOMATO, CK-7, S-100, glucagon, CD57, and a Ki-67 of 15%). Death was registered in two cases, both with bone metastases. Discussion: Some poor prognosis factors may be taken into account as lack of CHROMO immunostain, young age at diagnosis, genetic background, and lack of therapy options as surgery. Larger databases will provide more information. Conclusions: It is possible that the G2 NET group of tumors actually includes some different subtypes or in fact, a late diagnosis of the tumor might be associated with a poor diagnosis.

Corresponding author: Mara Carsote, MD; e-mail: carsote_m@hotmail.com

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