HPV and cervical squamous intraepithelial lesions: clinicopathological study

Vol. 52 No. 1 Suppl., 2011
This supplement was not sponsored by Outside Organizations.


Lavinia Mihaela Corneanu, Diana Stanculescu, Cecilia Corneanu

The aim of our study is to determine the evidence of HPV infection, by either HPV-DNA test or histopathological examination, in patients with abnormal PAP-smear, for further clinical management. In 6-month period, we retrospectively selected a number of 103 patients in 16-54 years range of age that were investigated by complex diagnostic techniques supporting the evidence of HPV infection. Initially, these patients were evaluated cytological using the Bethesda system for PAP-smear interpretation along with colposcopic examination. A number of these patients were orientated to HPV testing (PCR) or to biopsy with histopathological diagnostic intent. The net predominance of LSIL over HSIL cytodiagnostic class parallels with that of condyloma and CIN1 over CIN2 and CIN3 histodiagnostic and suggests that progression to high-grade intraepithelial squamous lesion is rare and the HPV infection remain mainly transitory. On the other side, the incidence and distribution of HPV types in cervical infections are high variable and may change over time, the cytological screening of sexual active female population remaining the most practical tool in detection of genital HPV infection.

Corresponding author: Lavinia Mihaela Corneanu, MD, e-mail: laviniadobrescu@ymail.com

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Irina Manoilescu, S. Teleman, Elena Cojocaru, Doina Mihaila, P. Plamadeala

Introduction: The need for reasoning with medical evidence the different types of shock, especially when there are medical and legal implications, has determined the search of biological markers of the shock. In the case of toxic septic shock, the most important markers to be used are: the cytokines, the tumor necrosis factor-alpha (TNF-alpha) and interleukin 6 (IL-6), procalcitonin, lactoferin and the vascular endothelial growth factor (VEGF). VEGF has an essential role in angiogenesis and vascular permeability. Materials and Methods: In our study group, we included 30 cases of different types of shock in which we studied the VEGF expression in the lungs. We added also 10 fragments of lung as control group. According to the etiology, the 30 cases of shock were: 15 with a toxic septic shock and 15 with a hemorrhagic shock. In all these cases we used the classical Hematoxylin and Eosin staining method and the immunohistochemical reactions for VEGF-A. Statistical analysis was performed using SPSS 13.0. Results: The VEGF expression was decreased in all the cases of toxic septic shock, in the endothelium and also in the alveolar epithelium, compared to a high level of expression in other cases of shock and in the control lung. Conclusions: These data allow us to appreciate that VEGF has a different expression in different types of shock and in the normal lung. We observed a statistically significant difference between VEGF expression in toxic septic shock and hemorrhagic shock (p=0.000001). There is a similarity of VEGF expression between hemorrhagic shock and the control lungs (p=0.00001). An obviously low VEGF expression in the toxic septic shock represents a useful biological marker in the forensic medical cases.

Corresponding author: Petru Plamadeala, MD, PhD, e-mail: p_petru@yahoo.com

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