Quantification of apoptotic phenomenon on endometrial biopsies in postmenopausal patients under hormonal replacement therapy (HRT)

Vol. 48 No. 2, 2007

ROMANIAN JOURNAL of MORPHOLOGY and EMBRYOLOGY

Dana Terzea, Cristina Iosif, Florina Vasilescu, F. Andrei, Camelia Dobrea, Alina Nicolae, Alina Georgescu, F. Mircea, M. Ceauşu, Ariana Vişan, Mihaela Mihai, Carmen Ardeleanu

Aim. To quantify the apoptotic phenomenon on endometrial biopsies in postmenopausal patients under hormonal replacement therapy (HRT). Material and methods. The study lot consisted of 30 endometrial biopsies on which we studied the apoptotic phenomenon through morphological and molecular biology techniques (TUNEL reaction). Examination of endometrial biopsies before and post-therapeutically has been made. Results and discussions. From morphological point of view, pre-therapeutically, endometrial biopsies presented apoptotic changes in about 1-3% of cells and under TSH, there have been observed apoptotic changes in about 1-2% of cells. In female reproductive system, we found out a raised rate of cellular proliferation and concurrently a raised rate of apoptosis. Apoptotic phenomenon can be observed in endometrium at every menstrual cycle. In proliferative endometrium apoptosis rate is low, but in endometrial carcinoma apoptosis rate grow up. Bcl2 and Bax are expressing in normal and hyperplastic endometrium, but in endometrial carcinoma Bcl2/Bax ratio decline. Conclusions. Quantification of apoptosis, using morphological and TUNEL reaction methods, on endometrial biopsies in postmenopausal patients before and after therapy indicate a low rate of apoptotic phenomenon.

Corresponding author: Dana Terzea, MD, e-mail: danaterzea@gmail.com

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