CD30 expression utilization for the accuracy of classical Hodgkin's lymphoma staging

Vol. 47 No. 2, 2006


Corina Flangea, Elena Potencz, Rodica Mihăescu, A. Anghel, S. Gîju, Marilena Motoc, C. Dogaru

Introduction. The presence of Reed-Sternberg malignant cells is absolutely necessary for Hodgkin's lymphoma diagnostic, but it is not always sufficient because can be observed Reed-Sternberg-like cells in other malignant and benignant diseases, too. The CD30 expression at Hodgkin and Reed-Sternberg level can give us supplementary information in differential diagnostic and can be used as progressive disease factor. Material and methods. Our study was composed from 63 cases histopathological diagnosticated with Hodgkin's lymphoma and hospitalized in Hematology Department of County Hospital Timisoara. CD30 expression was immunohistochemical semi-quantitative evaluated using clone BerH2 as primary antibody and APAAP-New Fuchsin as visualization system. Results and discussions. The increasing of CD30 expression occurs in the same time with advanced stages and the disease progression (p<=0.001). For I and II stages CD30 expression does not overcome (-/+) category while the III and IV stages, all the cases are situated in (+/-) and (+) categories. No connection can be noticed between histological type and CD30 expression (p<=1). We consider that using this staining, although less used in Romania, must be done in all Hodgkin's lymphoma and Hodgkin's lymphoma-like cases. We say that because the main cause of relapses is represented by inadequate clinical staging and diagnostic. Conclusions. In our study, the increasing of CD30 expression is associated with advanced disease stage. We recommend reinvestigating and restaging all cases that was included into an incipient stages and they have a CD30 expression situated in (+/-) and (+) intervals because some lymph nodes could be overlooked.

Corresponding author: Corina Flangea, MD, PhD, e-mail:

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